Ergothioneine Powder

Sourced and manufactured by Shaanxi Jiuyuan Biotechnology Co., Ltd., our premium Ergothioneine (EGT) powder is a naturally occurring amino acid derivative renowned for its potent antioxidant properties. Extracted or synthesized with meticulous care, EGT offers exceptional protection against oxidative stress and supports various health and wellness applications.

We offer two distinct grades to meet your specific formulation needs:

• Natural Source (1%): Extracted directly from natural sources, providing a touch of nature's inherent goodness.
• Fermentation Source (99%): Produced through advanced fermentation technology, ensuring high purity and consistent quality.

Ergothioneine is scientifically recognized for its ability to scavenge free radicals, support cellular health, and contribute to anti-aging effects. It finds versatile applications in nutraceuticals, cosmetics, and functional foods.

Shaanxi Jiuyuan Biotechnology is committed to delivering high-quality ingredients backed by rigorous quality control. Partner with us to access premium Ergothioneine powder and elevate your product offerings. Contact us today to learn more about our specifications and wholesale pricing.

I Natural Extraction Process of Ergothioneine

1. Raw Material Preparation: Selection of natural materials rich in Ergothioneine, such as edible fungi (e.g., Lentinula edodes (Shiitake), Pleurotus ostreatus (Oyster mushroom), Flammulina velutipes (Enoki)), animal liver, or kidney.

2. Extraction:

Solvent Extraction: The raw material is pulverized and mixed with an appropriate extraction solvent (e.g., 70% ethanol, acetone). The mixture is then subjected to stirring or ultrasonic treatment to dissolve the Ergothioneine from the raw material.

Hot Water Extraction: The raw material is mixed with water and heated to a specific temperature (e.g., 60°C to 80°C) for a defined period to dissolve the Ergothioneine into the water.

Separation: The extraction liquid is separated from the solid residue through centrifugation or filtration, yielding an Ergothioneine-containing extract.

3. Preliminary Purification:

Ultrafiltration: The extract is filtered using ultrafiltration membranes with a molecular weight cut-off (MWCO) of 1 kDa to 30 kDa to remove large molecular impurities.

Ion Exchange Chromatography: The ultrafiltrated extract is passed through an ion exchange resin column. Ergothioneine is preliminarily separated based on the differential adsorption of Ergothioneine and other impurities onto the resin.

4. Further Purification:

Silica Gel Column Chromatography: The solution from the ion exchange chromatography is subjected to silica gel column chromatography. Gradient elution is employed to further purify the Ergothioneine.

5. Drying and Packaging: The purified Ergothioneine solution is concentrated and then dried (e.g., freeze-drying or spray-drying) to obtain Ergothioneine powder, which is subsequently packaged.

II Fermentation and Purification Process of Ergothioneine

1. Strain Selection and Culture Medium Preparation: Selection of high Ergothioneine-producing strains, such as Pleurotus ostreatus or genetically engineered strains. Preparation of a suitable culture medium for strain growth and Ergothioneine synthesis, typically a liquid submerged fermentation medium.

Fermentation: The selected strain is inoculated into a fermenter, and fermentation is carried out under controlled conditions (e.g., temperature, pH, dissolved oxygen) on a large scale. Online monitoring and control systems are used to ensure stable fermentation conditions.

Harvesting: After fermentation, the mycelium is separated from the fermentation broth through centrifugation or filtration.

2. Preliminary Extraction:

Hot Water Extraction: The fermentation broth or mycelium is mixed with water and heated to a specific temperature to dissolve the Ergothioneine into the water.

Ultrafiltration: The hot water extract is filtered using ultrafiltration membranes to remove large molecular impurities.

3. Purification:

Ion Exchange Chromatography: The ultrafiltrated extract is passed through an ion exchange resin column to separate Ergothioneine based on the differential adsorption of Ergothioneine and other impurities onto the resin.

Chromatographic Purification: High-performance liquid chromatography (HPLC) or other chromatographic techniques are employed to further enhance the purity of Ergothioneine, achieving levels above 99%.

4. Drying and Packaging: The purified Ergothioneine solution is concentrated and then dried (e.g., freeze-drying or spray-drying) to obtain high-purity Ergothioneine powder, which is subsequently packaged.

III Comparison of the Two Processes

Natural Extraction:

Advantages: Natural raw materials, high product safety, no GMO risk.

Disadvantages: Low extraction efficiency, high cost, and difficult in scaling up for large-scale production.

Fermentation and Purification:

Advantages: High fermentation efficiency, scalable for large-scale production, low cost, high purity (can reach above 99%).

Disadvantages: Requires complex fermentation equipment and process control, high demands on strain and fermentation conditions.

The fermentation and purification process has become the primary method for Ergothioneine production due to its advantages in efficiency, cost-effectiveness, and high purity.

Antioxidant Activity: Ergothioneine is a highly effective antioxidant exhibiting a dual mechanism of action:

• Direct Free Radical Scavenging: EGT rapidly neutralizes a variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS), including superoxide anion (O2−​), hydrogen peroxide (H2​O2​), hydroxyl radical (⋅OH), and singlet oxygen (1O2​).
• Modulation of Antioxidant Defense Systems: EGT enhances cellular antioxidant capacity by activating the KEAP1-NRF2 signaling pathway, leading to the upregulation of downstream antioxidant genes such as heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO1), superoxide dismutase (SOD), and catalase (CAT).

Anti-Aging Effects: Ergothioneine contributes to the delay of aging through the following mechanisms:

• Maintenance of Genomic Stability: EGT protects cells from oxidative damage, thereby preserving the integrity of the genome.
• Regulation of Epigenetic Modifications: By influencing epigenetic modifications, EGT modulates gene expression patterns associated with the aging process.
• Extension of Healthspan: Studies have demonstrated that Ergothioneine can extend both the lifespan and healthspan of aged nematodes and rats, accompanied by improvements in motor function and stress resistance.

Anti-Photoaging Effects: Ergothioneine exerts protective effects on the skin:

• Ultraviolet (UV) Radiation Absorption: EGT absorbs UV radiation in the range of 310-360 nm, mitigating UV-induced skin damage.
• Inhibition of Collagen Degradation: By inhibiting the activity of matrix metalloproteinase-1 (MMP-1), EGT reduces collagen breakdown while concurrently promoting collagen synthesis.
• Repair of Skin Damage: Activation of the Nrf2 pathway by EGT facilitates the repair of the skin barrier compromised by UV irradiation and reduces erythema.

This naturally occurring amino acid derivative exhibits a broad spectrum of applications, supported by scientific evidence, across various sectors:

1. Food and Nutritional Supplements: Its antioxidative properties enable the inhibition of polyphenol oxidase activity, mitigating oxidative deterioration in food during processing and storage. For instance, its application in shrimp and crab preservation effectively prevents melanosis, extending shelf life. Furthermore, research indicates its capacity to significantly extend the lifespan and enhance the motor skills and stress resilience of aged nematodes and rodents, concurrent with elevated NAD⁺ levels and improved mitochondrial function. As a natural amino acid derivative, this compound serves as a valuable nutritional supplement, contributing to the body's antioxidant defense mechanisms.
2. Cosmetics and Personal Care Products: Applications in this domain are primarily attributed to its antioxidant and anti-photoaging characteristics. The compound demonstrates the ability to absorb ultraviolet radiation, thereby reducing UV-induced oxidative stress and protecting the skin from photoaging. Moreover, by inhibiting the activity of collagen-degrading enzymes such as MMP-1, it can diminish collagen loss while promoting its synthesis, leading to improved skin elasticity. Additionally, its capacity to activate the Nrf2 signaling pathway facilitates the repair and regeneration of skin cells.
3. Medical and Health Fields: This molecule contributes to cardiovascular health by mitigating oxidative stress and potentially lowering the risk of cardiovascular diseases. Studies have shown an inverse correlation between its levels and the incidence of coronary artery disease, cardiovascular mortality, and all-cause mortality. In diabetes management, supplementation can reduce oxidative stress and inflammatory markers in patients, alongside improving vascular endothelial function. Its ability to permeate the blood-brain barrier suggests a neuroprotective role, potentially offering therapeutic avenues for neurodegenerative conditions like Alzheimer's disease. Furthermore, it may alleviate the side effects of chemotherapy, such as peripheral neuropathy, and potentially enhance the efficacy of cancer immunotherapy.
4. Animal Health and Aquaculture: In aquaculture, this ingredient is incorporated into feed for shrimp and crabs to prevent melanosis and extend their marketability. Animal models have demonstrated its efficacy in improving muscle quality, endurance, and overall exercise capacity.
5. Other Applications: Animal studies suggest a role in preventing pregnancy-induced hypertension (preeclampsia) by reducing associated hypertension, mitigating oxidative stress, and improving fetal development. Additionally, it finds utility as a food preservative in meat and seafood products, reducing oxidative spoilage.

In conclusion, this natural compound holds significant promise across the food, cosmetic, medical, and animal health industries, with its diverse health benefits supported by a growing body of scientific research.

Established in 2012, Shaanxi Jiuyuan Biotechnology is a modern, high-tech enterprise specializing in the R&D, production, and sales of premium plant extracts and fruit & vegetable powders. Located in the Qinling Mountains region, renowned for its rich medicinal resources, we ensure quality from the source.

Our state-of-the-art 70,000 sqm facility features advanced production lines and a Class 100,000 cleanroom, boasting an annual capacity of over 6,000 tons to meet diverse needs. We are committed to stringent quality control, ensuring full product traceability and holding numerous international certifications, including cGMP, EU/USDA Organic, ISO, FSSC 22000, HACCP, Kosher, Halal, and FDA registration.

With a strong focus on R&D and innovation, supported by collaborations with leading institutions and our new, well-equipped R&D and testing center (operational April 2025), we continuously develop high-quality, reliable ingredients.

Choose Jiuyuan for consistent quality, comprehensive certifications, and strong production capacity. Let's build a mutually successful partnership.

Pls feel free to contact us for any needs!

Email: info@jiuybiotech.com

Phone No: +86 173 00897248

We cooperate with multiple professional logistics companies to provide efficient and secure logistics services.
We ensure the timely and complete delivery of products to customers, regardless of their location.
We offer a variety of logistics options for customers to choose from, including express delivery, air freight, and sea freight.

Storage: Keep in a cool, dry, and clean place, protect from moisture and direct light.
Bulk Package: 25kg/drum.
Lead Time: 7 days after your order.
Shelf Life: 2 years.
Remark: Customized specifications can also be achieved.