Bitter Melon Peptide

Sourced from high-quality Momordica charantia, our Bitter Melon Peptide is a naturally derived ingredient rich in bioactive peptides. Extracted and purified through an advanced enzymatic process, this low molecular weight protein offers superior bioavailability and a range of health benefits.

Key advantages include its potent ability to support healthy blood glucose levels by mimicking insulin action and improving insulin sensitivity. It also aids in metabolic regulation, potentially assisting in weight management. Furthermore, its antioxidant and anti-inflammatory properties contribute to overall well-being.

Our Bitter Melon Peptide is a versatile ingredient suitable for dietary supplements, functional foods, and beverages. Backed by scientific research, it meets stringent European quality standards. Offer your customers a natural and effective solution for glucose control and metabolic health. Contact us for bulk supply and partnership opportunities.

1. Raw Material Preparation

• Cleaning: Bitter melon raw materials (fresh or dried bitter melon, bitter melon seeds) are initially cleaned with tap water, followed by high-concentration electrolytic ozone water at an ozone concentration of 10-20 mg/L and a water flow rate of 150-450 L/h.
• Pulping and Crushing: The cleaned bitter melon raw materials are then pulped and crushed.

2. Extraction

• Purified Water Addition: 5-10 times the mass of purified water, with the system pH adjusted to near neutral using a buffer salt system, is added to the crushed or pulped bitter melon material.
• Ultrasound-Assisted Countercurrent Extraction: Ultrasound-assisted countercurrent extraction is performed 2-4 times at 38-52°C, with each extraction lasting 30-60 minutes.

3. Centrifugation for Residue Removal

• Centrifugation: A disc stack centrifuge or tubular centrifuge is used to remove impurities, yielding a centrifugal liquid.

4. Microfiltration Membrane Filtration

• Microfiltration Membrane System: The centrifugal liquid is filtered through a microfiltration membrane system with a molecular weight cut-off of 0.1μm to 3μm to remove large molecular impurities, resulting in microfiltration permeate.
• Operating Conditions: The operating temperature is 15-35°C, and the operating pressure is 0.1-0.5 MPa.

5. Ultrafiltration Membrane Purification

• First Ultrafiltration: The microfiltration permeate is filtered through a spiral-wound ultrafiltration membrane purification system with a molecular weight cut-off of 50-200 kDa, yielding ultrafiltration permeate.
• Second Ultrafiltration: The ultrafiltration permeate from the first stage is further filtered through a spiral-wound ultrafiltration membrane purification system with a molecular weight cut-off of 500-1000 Da, resulting in ultrafiltration retentate.
• Operating Conditions: The operating temperature is 15-35°C, and the operating pressure is 0.3-0.8 MPa.

6. High-Pressure Reverse Osmosis Membrane Concentration

• Reverse Osmosis Membrane System: The ultrafiltration retentate is concentrated using a spiral-wound high-pressure reverse osmosis membrane system with a molecular weight cut-off of 100-150 Da to remove water and some residual inorganic salts and small molecular impurities, resulting in a concentrated liquid with a solid content ≥30%.
• Operating Conditions: The operating temperature is 15-35°C, and the operating pressure is 3.5-5.5 MPa.

7. Drying

• Drying Method: The concentrated liquid is dried to obtain bitter melon extract powder with a polypeptide protein content of no less than 25%.
• Drying Technique: Low-temperature spray drying (flash drying) is employed.

8. Enzymatic Hydrolysis

• Enzyme Hydrolysate Preparation: Bitter melon powder is mixed with water, the pH is adjusted to 8-9, and the mixture is soaked for 15-18 hours. Solid-liquid separation is performed to obtain the supernatant. The supernatant's pH is adjusted to 4-6 for precipitation, followed by solid-liquid separation to collect the solid portion. The solid portion is then mixed with water, heated at 85-100°C for 20-30 minutes, and homogenized to obtain a suspension.
• Enzymatic Reaction: The suspension's pH is adjusted to 5-8, and a composite enzyme preparation consisting of flavorzyme, papain, and pepsin is added for enzymatic hydrolysis at a temperature of 45-65°C for 2-6 hours.

9. Enzyme Inactivation

Enzyme Inactivation Conditions: The enzyme hydrolysate undergoes enzyme inactivation treatment at a temperature of 95-125°C for 0.5-2 hours.

10. Purification

• Filtration and Spray Drying: The enzyme-inactivated hydrolysate is centrifuged, and the supernatant is first filtered through a plate and frame filter press, followed by ceramic membrane filtration, yielding active peptide permeate. This permeate is then concentrated and spray-dried to obtain bitter melon active peptide powder.
• Spray Drying Conditions: Spray drying is performed at 80-105°C.

11. Quality Control

• Purity Detection: Bitter melon peptide is purified using ion exchange chromatography, gel filtration chromatography, or other appropriate methods to ensure a polypeptide protein content of no less than 25% and a polypeptide protein molecular weight distribution between 4.5-68 kDa.
• Lyophilization or Spray Drying: The purified bitter melon peptide powder is obtained through lyophilization or spray drying.

• Natural & Bioavailable: Derived from bitter melon, our peptide is a natural, small molecule protein with potential for enhanced absorption.
• Blood Sugar Support: May help regulate blood glucose by mimicking insulin action, improving insulin sensitivity, and slowing sugar absorption. Initial studies show potential benefits for fasting glucose and HbA1c.
• Metabolic & Weight Management Potential: May influence fat metabolism and promote satiety, potentially aiding in weight management.
• Antioxidant & Anti-inflammatory: Contains compounds that may combat oxidative stress and reduce inflammation.
• Cardiovascular Health: Preliminary research suggests potential benefits for cholesterol levels and blood pressure regulation.
• Other Potential Benefits: May support immune function, improve digestion, and offer liver protection (further research needed).
• Safe & Suitable: Generally well-tolerated and potentially beneficial for those seeking blood sugar and weight management support.
• Convenient Use: Available in easy-to-consume formats like capsules and powders. Preliminary data suggests taking before meals may be optimal.

Pharmaceuticals:

• Glycemic Control: Demonstrates potential in managing blood glucose by mimicking insulin action and enhancing glucose uptake, with studies suggesting benefits in diabetes management.
• Antihypertensive Potential: Certain components may inhibit ACE activity, showing promise in lowering blood pressure (primarily in animal models with specific peptides).
• Anti-tumor Potential: In vitro studies indicate inhibitory effects on the proliferation and induction of apoptosis in certain cancer cell lines.
• Immunomodulation: May activate macrophages and T lymphocytes, potentially enhancing immune function.

Food Science:

• Nutraceuticals/Dietary Supplements: Used in products aimed at blood sugar regulation and immune support.
• Functional Beverages: Incorporated to enhance the health profile of beverages.
• Cosmetics & Personal Care:
• Anti-aging: Antioxidant properties suggest potential in anti-aging formulations and skin protection.
• Skin Care: Investigated for potential use in addressing skin conditions like eczema and psoriasis.

Other Areas:

• Anti-inflammatory & Antioxidant: Its properties suggest potential in cardiovascular disease prevention.
• Digestive Health: May promote digestion and improve gastrointestinal function.

Scientific Basis:

Clinical studies indicate potential for lowering fasting blood glucose and HbA1c in diabetic individuals. Animal studies suggest potential in preventing and treating conditions like diabetes, hypertension, and certain cancers.

Established in 2012, Shaanxi Jiuyuan Biotechnology is a modern, high-tech enterprise specializing in the R&D, production, and sales of premium plant extracts and fruit & vegetable powders. Located in the Qinling Mountains region, renowned for its rich medicinal resources, we ensure quality from the source.

Our state-of-the-art 70,000 sqm facility features advanced production lines and a Class 100,000 cleanroom, boasting an annual capacity of over 6,000 tons to meet diverse needs. We are committed to stringent quality control, ensuring full product traceability and holding numerous international certifications, including cGMP, EU/USDA Organic, ISO, FSSC 22000, HACCP, Kosher, Halal, and FDA registration.

With a strong focus on R&D and innovation, supported by collaborations with leading institutions and our new, well-equipped R&D and testing center (operational April 2025), we continuously develop high-quality, reliable ingredients.

Choose Jiuyuan for consistent quality, comprehensive certifications, and strong production capacity. Let's build a mutually successful partnership.

Pls feel free to contact us for any needs!

Email: elsa.marketing@jiuybiotech.com

Phone No: +86 173 00897248

We cooperate with multiple professional logistics companies to provide efficient and secure logistics services.
We ensure the timely and complete delivery of products to customers, regardless of their location.
We offer a variety of logistics options for customers to choose from, including express delivery, air freight, and sea freight.

Storage: Keep in a cool, dry, and clean place, protect from moisture and direct light.
Bulk Package: 25kg/drum.
Lead Time: 7 days after your order.
Shelf Life: 2 years.
Remark: Customized specifications can also be achieved.

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