Natto Fermented Powder

Natto Fermented Powder is produced through solid-state or submerged fermentation of non-GMO soybeans using Bacillus subtilis natto. The resulting serine protease - nattokinase (NK) - is extracted, purified, and spray-dried into a stable, odor-neutralized powder. Each gram delivers a precisely standardized enzyme activity measured in Fibrinolytic Units (FU), the internationally accepted benchmark for nattokinase potency.
At Jiuyuan Bio, we supply Natto Fermented Powder across a full activity range - from 2,000 FU·g⁻¹ for entry-level formulations to 20,000 FU·g⁻¹ for high-potency cardiovascular products. Every batch is verified by the JHFA fibrin plate method at 37°C, pH 7.4 - the gold standard for nattokinase activity testing.

Standard Grades

High-Purity Crystalline Grade

For pharmaceutical and clinical research applications, we offer a crystalline-grade nattokinase with:

• Nattokinase purity ≥80% (batch crystallization process)
• Verified by SDS-PAGE + HPLC size-exclusion chromatography
• Suitable for API-level formulation and pharmacokinetic studies

Transparency in production is non-negotiable for regulated markets. Here's how our Natto Fermented Powder is made:

1. Raw Material Selection Non-GMO soybeans sourced from Heilongjiang province - China's premier non-GMO soybean belt. Each incoming lot undergoes PCR-based GMO screening and allergen verification before entering production.

2. Strain Preparation Authenticated Bacillus subtilis natto seed culture is prepared under controlled conditions. Strain identity is confirmed by 16S rRNA sequencing for every production cycle.

3. Fermentation

• Solid-state fermentation: Traditional method; higher enzyme expression per gram of substrate
• Submerged liquid fermentation: Scalable, continuous production; tighter batch-to-batch consistency
• Fermentation parameters - dissolved oxygen, pH, temperature - are monitored in real time via in-line sensors

4. Extraction and Purification Fermented biomass undergoes aqueous extraction followed by multi-stage membrane filtration (ultrafiltration + nanofiltration). This step removes Bacillus cells, polyglutamic acid (PGA), and residual soy matrix.

5. Vitamin K2 Removal (Optional) For markets requiring K2-free product - particularly for customers on anticoagulant therapy - an additional adsorption step eliminates vitamin K2 to below detectable limits.

6. Spray Drying and Deodorization The purified enzyme solution undergoes spray drying with food-grade excipients. A deodorization step eliminates the characteristic natto odor - critical for consumer-facing supplement formats.

7. Standardization and Blending Final powder is blended to target FU activity. Every batch is assayed by the JHFA fibrin plate method before release.

8. Packaging 25 kg fiberboard drums with double PE inner bags and desiccant sachets. Cold-chain shipment at 2–8°C is recommended for orders above 100 kg.

Natto Fermented Powder is not a single-compound ingredient. Understanding its full biochemical profile helps formulators make smarter decisions.

Primary Active Enzyme: Nattokinase

• Class: Serine protease (subtilisin family)
• Structure: Single-chain polypeptide, 275 amino acids
• Molecular weight: 27.7 kDa
• Activity unit: FU (Fibrinolytic Unit) - defined as the enzyme quantity that dissolves 1 μmol of fibrin per minute at 37°C, pH 7.4
• Mechanism: Direct fibrin cleavage + activation of endogenous tissue plasminogen activator (t-PA) system

Co-occurring Bioactives

Why FU Matters More Than Milligrams

This is a point many buyers miss. Milligram dosage is meaningless for enzyme ingredients. A product labeled "500 mg nattokinase" tells you nothing about potency. What matters is the FU activity per serving.

The research-supported dose range for cardiovascular applications is 2,000–4,000 FU per day, as referenced in the landmark NATTOPHARMA-funded clinical trials and the 2021 meta-analysis published in Nutrients (Weng et al., 2021).

Always specify FU activity - not milligrams - in your product specifications and label claims.

Potential Fibrinolytic Activity

Nattokinase, an enzyme present in Natto powder, has demonstrated in vitro and some in vivo evidence of fibrinolytic activity, suggesting a potential role in the breakdown of fibrin, a component of blood clots. Further well-controlled human studies are needed to fully elucidate its efficacy, speed of action, and duration in various physiological contexts. While it may influence thrombus-decomposing enzymes, more research is required to confirm the specific mechanisms and clinical significance of these interactions.

Potential Role in Blood Pressure Management:

Some research suggests that long-term consumption of nattokinase may contribute to modest reductions in blood pressure in individuals with hypertension. However, the evidence is not conclusive, and the magnitude of the effect can vary. Individuals with high blood pressure should consult with healthcare professionals for appropriate management strategies.

Antioxidant Properties:

Natto powder contains compounds like isoflavones and vitamin E, which exhibit antioxidant activity in vitro by neutralizing free radicals. The extent to which these properties provide significant protection against oxidative stress and support cellular health in vivo requires further investigation in human studies.

Potential Influence on Blood Lipids:

Preliminary studies indicate that components in Natto powder may have a role in modulating blood lipid profiles, potentially contributing to lower LDL cholesterol and increased HDL cholesterol levels. However, more robust clinical trials are necessary to confirm these effects and their clinical relevance.

Potential Support for Digestive Health:

The presence of Bacillus subtilis var. natto spores in Natto powder may contribute to gut health by potentially acting as a probiotic, helping to maintain intestinal balance. Further research is needed to determine the extent of its impact on digestive and immune function.

Dietary Supplement Industry

Health Care Products Industry

Food Industry and Biotechnology 

Finished Dosage Form Recommendations

Format	Recommended Activity	Key Formulation Notes	Target Market
Enteric-coated softgel	2,000–5,000 FU	Lecithin + beeswax matrix; acid-resistant shell	US, EU, AU
Chewable tablet	1,000–2,000 FU	Xylitol + berry powder; low-temperature compression	Canada, Japan
Stick pack (powder)	5,000 FU	Warm water dissolution; FOS prebiotic blend	Japan, South Korea
Hard capsule (enteric)	2,000–10,000 FU	HPMC enteric shell; K2-free grade preferred	EU Novel Food pathway
Pet nutrition powder	10,000 FU	Yeast hydrolysate blend; mix-in format	US (AAFCO), EU
Functional beverage	2,000 FU	Requires microencapsulation for stability	Emerging markets

Market-Specific Claim Guidance

• United States (DSHEA)

Permitted structure/function claim: "Supports healthy blood flow and cardiovascular function"

FU activity must appear on Supplement Facts panel

No drug claims permitted

• European Union

Novel Food status applies - EU Novel Food authorization or substantial equivalence documentation required

EFSA Article 13.1 positive list references available for ACE inhibition claims

• Japan

Eligible for FOSHU (Foods for Specified Health Uses) certification: "Helps maintain intestinal environment"

Functional Labeling Food (機能性表示食品) pathway available with supporting literature

• Canada (NNHPD)

Traditional use claim pathway available

Product license required before market entry

• China (Blue Hat)

Applicable health function: "Auxiliary protection against chemically induced liver injury"

Registered health food pathway under NMPA

Our quality assurance framework covers every stage from raw material intake to finished goods release.

Incoming Material Testing

• Non-GMO verification by PCR (polymerase chain reaction)
• Allergen screening (soy protein quantification)
• Heavy metal profiling: Pb, As, Cd, Hg - USP <231> limits
• Pesticide residue panel: EU MRL compliance

In-Process Controls

• Real-time dissolved oxygen monitoring during fermentation
• Enzyme activity curve tracking throughout fermentation cycle
• Membrane filtration integrity testing at each purification stage

Finished Product Release Testing

Facility Standards

• GMP-certified production facility
• Clean room classification: Grade C + Grade A filling lines
• Annual third-party facility audits available upon request

The following documentation is available for qualified buyers:

✅ Certificate of Analysis (CoA) - batch-specific

✅ Material Safety Data Sheet (MSDS)

✅ Technical Data Sheet (TDS)

✅ Heavy Metal Test Report (ICP-MS, third-party)

✅ Microbiological Test Report

✅ Non-GMO Certificate (PCR-based)

✅ Allergen Declaration

✅ ISO 9001 Quality Management Certificate

✅ HACCP Certificate

✅ Kosher Certificate

✅ Halal Certificate

✅ Stability Data (accelerated + real-time)

✅ EU Novel Food supporting dossier (on request)

All documents are provided in English. Chinese-language originals available upon request for customs clearance purposes.

Nattokinase vs. Competing Fibrinolytic Ingredients

Key takeaway: Nattokinase occupies a unique position - it's the only orally delivered enzyme with both direct fibrinolytic activity and a credible human clinical evidence base, within the dietary supplement regulatory framework.

Natto Fermented Powder Grades: Which One Do You Need?

Our nattokinase is derived from carefully selected high-quality natto, which is naturally fermented by Bacillus natto to ensure the purity and naturalness of the raw materials. Advanced biotechnology ensures that each batch of products meets the highest quality standards. Industry-leading extraction technology is used to ensure the high purity and biological activity of the product. We always put the needs of our customers first. Our sales and customer service teams are always ready to provide you with professional advice and thoughtful service.

Here are our packaging and shipping methods for your reference:

Why Choose US?

1. Selected Raw Materials
Our plant extracts are sourced from premium plants from all over China, grown in a pollution-free environment to obtain the purest, highest quality raw materials.
2. Cutting-edge technology & superior craftsmanship
Adopt advanced extraction technology and strict production processes, including fermentation, organic solvent extraction, freeze drying, microwave-assisted extraction, etc., to maximize the retention of active ingredients in plants. The production process complies with GMP standards to ensure product consistency and reliability.

3. Authoritative certification
Soybean Natto Powder's safety and effectiveness have been proven through extensive scientific research and third-party authoritative certification. The active ingredient content will be marked on the product label for your reference.

4. Quick response & stable supply
We promise to respond quickly to your order needs and ensure that your orders can be delivered in a timely and safe manner through an efficient logistics system. Strong production capacity can ensure a stable supply of products.
5. Customer First
We always put customer needs first. Our sales and customer service teams are always ready to provide you with professional advice and thoughtful service. We believe that good service is the cornerstone of building a long-term cooperative relationship.

Who Buys Natto Fermented Powder?

Our customers include:

• Nutraceutical brands formulating cardiovascular, healthy aging, and metabolic health products
• Contract manufacturers (CMOs) needing consistent, documentation-ready enzyme ingredients
• Private label operators building premium supplement lines for US, EU, and APAC markets
• Functional food developers exploring enzyme-fortified beverages and stick packs
• Veterinary supplement brands targeting pet cardiovascular health

Common Sourcing Pain Points - Solved

"I can't verify the actual enzyme activity." Every batch ships with a JHFA-method CoA and third-party lab confirmation. We welcome independent re-testing.

"The last supplier's product had a strong natto odor." Our deodorization process eliminates the characteristic fermentation odor. Suitable for consumer-facing formats without masking agents.

"I need K2-free product for anticoagulant-therapy customers." Our K2-removal process brings vitamin K2 below detectable limits. Full HPLC documentation provided.

"Regulatory compliance documentation is always incomplete." We maintain a pre-built regulatory dossier covering US, EU, Canada, Japan, and Australia. Most documents are available within 24 hours of request.

"MOQ is too high for trial orders." We offer sample quantities starting from 100g for qualification testing, with no commitment required.

Q1: What is the difference between Natto Fermented Powder and nattokinase extract?

Natto Fermented Powder is the broader category - it can refer to whole-ferment powder (containing Bacillus cells, PGA, K2, and nattokinase) or purified nattokinase extract. Our product is a purified, standardized nattokinase extract in powder form, not a whole-food ferment. This distinction matters for dosing accuracy, label claims, and regulatory compliance.

Q2: Why does nattokinase need enteric delivery?

Nattokinase is a protein-based enzyme. Gastric acid (pH ~1.5–2.5) rapidly denatures it before it reaches the small intestine. Enteric coating - whether on softgels or hard capsules - protects the enzyme through the stomach and releases it at intestinal pH (≥6.0), where absorption occurs. Formulating without enteric protection significantly compromises bioavailability.

Q3: How should FU activity be declared on the label?

In the US, the FDA expects enzyme activities to appear on the Supplement Facts panel in the unit relevant to the enzyme's function. For nattokinase, FU (Fibrinolytic Units) is the accepted unit. Declaring only milligrams without FU activity is considered insufficient for consumer transparency and may draw regulatory scrutiny.

Q4: Is your nattokinase derived from non-GMO soybeans?

Yes. All production batches use non-GMO soybeans sourced from Heilongjiang province, China. Non-GMO status is verified by PCR testing on every incoming raw material lot. Non-GMO certificates are available with each shipment.

Q5: Can nattokinase be combined with other cardiovascular ingredients?

Yes - and it's often more effective in combination. Well-documented synergistic pairings include:

Red yeast rice (monacolin K): Complementary lipid-lowering + fibrinolytic action

Coenzyme Q10: Mitochondrial support alongside vascular health

Omega-3 fatty acids: Antiplatelet + fibrinolytic dual mechanism

Magnesium: Vascular tone regulation

Note: Customers taking warfarin or other anticoagulants should consult a physician before use. Use K2-free grade to eliminate any potential vitamin K interaction.

Q6: What is the recommended storage condition during shipping?

For short-distance or air freight under 72 hours, ambient temperature with desiccant is acceptable for the 2,000–5,000 FU grades. For bulk sea freight or high-activity grades (≥10,000 FU), cold-chain shipping at 2–8°C is strongly recommended to preserve enzyme activity over transit. We provide stability data to support your logistics planning.

Q7: Do you offer white-label or custom-specification production?

Yes. We support:

Custom FU activity (any grade between 2,000–20,000 FU·g⁻¹)

Custom particle size and flowability specifications

K2-free or K2-retained grades

Organic-certified substrate options (on request)

Private label documentation including custom CoA headers

Contact our technical team to discuss your specification requirements.

Q8: What regulatory pathway applies for selling nattokinase in the EU?

Nattokinase falls under EU Novel Food Regulation (EU) 2015/2283 because it was not consumed to a significant degree in the EU before May 1997. Brands selling nattokinase supplements in the EU must either hold a Novel Food authorization or demonstrate substantial equivalence to an authorized product. We can provide supporting technical documentation to assist your regulatory submission.

Jiuyuan Bio supplies Natto Fermented Powder to nutraceutical brands, CMOs, and private label operators across North America, Europe, and Asia-Pacific. Our enzyme is standardized, fully documented, and ready for regulated markets.

Whether you need a 100g sample for lab qualification or a 500 kg bulk order for production, our team responds within one business day.

📩 Contact us: info@jiuybiotech.com 🌐 Explore our full catalog: jiuyuanbio.com

Request a sample, CoA, or technical dossier - we'll get back to you within 24 hours.