Vitamin K2 Powder

Vitamin K2 is a fat-soluble nutrient essential for regulating calcium metabolism, supporting cardiovascular health, and maintaining strong bones. With increasing global demand for bone health and heart-support supplements, K2 remains one of the fastest-growing nutraceutical ingredients.

Shaanxi Jiuyuan Biotechnology, with 13+ years of professional manufacturing experience, offers high-purity Natural Vitamin K2 Powder (MK-7 / MK-4) produced via advanced fermentation of Bacillus subtilis natto. Our products provide:

• Excellent stability and high bioavailability
• Microencapsulated options for tablets and capsules
• Flexible assay range: 0.2%–5%

Our K2 powders meet international regulatory standards for the United States and EU, supporting B2B customers in dietary supplement, functional food, and nutraceutical industries.

What Is Natural Vitamin K2 Powder?

Vitamin K2 (menaquinone) includes multiple subtypes, the most commonly used being MK-7 and MK-4.

• MK-7: Long half-life, excellent bioavailability, ideal for dietary supplements.
• MK-4: Shorter half-life, widely used in fortified foods and nutrition premixes.

Our K2 powder is 100% fermentation-derived, non-synthetic, non-GMO, and allergen-free, ensuring a clean label ingredient for functional food brands worldwide.

COA, MSDS, Allergen Statement, and Manufacturing Flow Chart available upon request.

• Supports Calcium Regulation

Activates osteocalcin and matrix GLA protein (MGP) to direct calcium to bones and prevent arterial calcification.

• Promotes Bone Strength

Clinical studies show K2 enhances bone mineral density and reduces age-related bone loss.

• Supports Cardiovascular Health

Helps prevent arterial stiffness by ensuring calcium is deposited in bones rather than arteries.

• Enhances Nutrient Synergy

Often combined with Vitamin D3, Magnesium, and Calcium in bone-health formulations for improved efficacy.

• Long Half-Life (MK-7)

Enables once-daily, low-dose formulations ideal for premium dietary supplements.

(References included at the bottom for each claim; see "Scientific References" section)

Natural vitamin K2 production is fundamentally different from standard botanical extraction - it is a precision fermentation process where microbial strain selection, fermentation parameters, and downstream purification all critically determine the trans-isomer purity and biological activity of the finished ingredient.

Stage 1 - Fermentation Strain Selection & Seed Culture MK-7 production begins with authenticated Bacillus subtilis natto strains - the same bacterial species responsible for traditional Japanese natto fermentation. Strain identity and MK-7 productivity are verified before each production run. Seed cultures are prepared under controlled conditions to ensure consistent inoculum quality.

Stage 2 - Submerged Fermentation B. subtilis natto is cultivated in submerged fermentation vessels under tightly controlled parameters: temperature (37°C ± 1°C), pH, dissolved oxygen, agitation rate, and nutrient feed composition. MK-7 accumulates intracellularly and in the fermentation broth. Fermentation duration and harvest timing are optimized to maximize MK-7 yield and trans-isomer proportion. Soybean-based fermentation substrates are used - the source of the soy allergen declaration required for finished product labeling.

Stage 3 - Biomass Harvest & Cell Disruption Fermentation broth is harvested at peak MK-7 titer. Bacterial cells are separated and disrupted to release intracellular MK-7.

Stage 4 - Extraction & Purification MK-7 is extracted from the disrupted biomass using food-grade organic solvents. The crude extract undergoes multi-stage purification - including liquid-liquid partition, column chromatography, and crystallization - to achieve target purity and trans-isomer specification. Each purification stage is monitored by HPLC for MK-7 content and trans/cis isomer ratio.

Stage 5 - Spray Drying with Carrier Encapsulation Purified MK-7 is blended with food-grade carrier (maltodextrin or acacia gum) and spray-dried to produce a free-flowing, light-stable powder. Carrier encapsulation is critical for MK-7 stability - the vitamin is highly susceptible to photodegradation and oxidative degradation without protective matrix. Encapsulation also improves dispersibility in aqueous formulation environments.

Stage 6 - QC Testing & Batch Release Every batch undergoes full in-house and third-party QC testing - including HPLC for MK-7 assay and trans/cis isomer ratio, soy allergen ELISA, and complete safety parameter testing - before release.

Stage 7 - Packaging Finished powder is nitrogen-flushed and sealed in foil-laminated bags. Light-protective, oxygen-barrier packaging is mandatory for vitamin K2 stability during storage and transit.

Vitamin K2's pharmacological profile is mechanistically precise - it operates through a well-characterized biochemical pathway with direct clinical consequences. Here is the science behind the supplement:

MK-7 vs. MK-4 - The Pharmacokinetic Distinction That Matters:

The difference between MK-7 and MK-4 is not merely structural - it is pharmacokinetically decisive for supplement formulation:

• MK-7's 72-hour plasma half-life means a single daily dose maintains effective circulating levels for carboxylation of osteocalcin and MGP throughout the day and night - a pharmacokinetic advantage that MK-4 cannot replicate at comparable doses. This is why MK-7 has become the dominant form in Western supplement markets.
• Bone Health Mechanism in Detail: Published RCT data confirms that MK-7 supplementation at 180 mcg/day for 3 years significantly increased bone mineral content and density at the lumbar spine and femoral neck in postmenopausal women, while simultaneously improving bone strength indices. The mechanism operates through carboxylation of osteocalcin, enabling it to bind calcium ions and incorporate them into the hydroxyapatite crystal lattice of bone.
• Cardiovascular Mechanism in Detail: MGP is the most potent known inhibitor of vascular calcification. In its uncarboxylated (inactive) form - which predominates in vitamin K2-insufficient individuals - MGP cannot bind calcium in arterial walls, allowing progressive calcification of the arterial media. Vitamin K2 supplementation activates MGP through carboxylation, restoring its calcification-inhibitory function. Published clinical data demonstrates significant reductions in dp-ucMGP (the biomarker of inactive MGP) and improvements in arterial stiffness indices following K2 supplementation.

The Vitamin D3 + K2 combination deserves particular attention: vitamin D3 increases calcium absorption from the gut, while vitamin K2 directs that absorbed calcium to bone rather than soft tissue. The two nutrients are mechanistically synergistic - and the D3+K2 combination product category has become one of the fastest-growing segments in the bone health supplement market globally.

Natural vitamin K2 production involves unique quality control challenges - particularly around trans-isomer verification, soy allergen management, and photostability - that require specialized analytical protocols beyond standard supplement QC:

Fermentation Process Controls

• Authenticated B. subtilis natto strain verification before each production run
• Real-time fermentation parameter monitoring (temperature, pH, dissolved oxygen)
• In-process HPLC monitoring of MK-7 titer and trans/cis isomer ratio during fermentation
• Harvest timing optimization based on peak MK-7 productivity data

Purification Process Controls

• HPLC monitoring of trans-MK-7 content at each purification stage
• Solvent residue testing post-extraction (ICH Q3C compliant)
• Yield tracking against validated process benchmarks

Finished Product Release Testing

• HPLC assay for MK-7 content (primary potency marker)
• HPLC trans/cis isomer ratio verification (≥97% all-trans required)
• Soy allergen testing by ELISA (mandatory - natto fermentation substrate)
• Full heavy metals panel (ICP-MS)
• Pesticide residue screening (GC/LC-MS)
• Residual solvents (GC, ICH Q3C compliant)
• Microbial enumeration and pathogen absence
• Physical parameter verification (appearance, color, particle size, moisture)

Photostability Testing

Vitamin K2 is highly photolabile. Finished product photostability is verified under ICH Q1B conditions. Light-protective packaging is mandatory for all shipments.

Third-Party Verification

All production batches are available for independent third-party testing through SGS, Eurofins, or equivalent ISO/IEC 17025-accredited laboratories. Split samples for pre-shipment verification are provided upon request.

Soy allergen management: Natto fermentation uses soybean-based substrates, making soy allergen declaration mandatory for MK-7 products in most regulated markets. We perform ELISA-based soy allergen testing on every finished batch and provide written allergen statements with each shipment. This is a non-negotiable compliance requirement - not a discretionary label element.

• MOQ: 1 kg (customizable for bulk orders)
• Lead Time: 5–7 days for ≤1000 kg air shipment; ~30 days for sea freight >300 kg
• COA Availability: Certificate of Analysis provided for each batch
• Third-Party Testing: Optional on request for purity, Trans-MK-7, microbial limits
• Regulatory Support: Compliant with US/EU standards; cGMP, ISO, HACCP, FSSC 22000, Halal, Kosher
• Custom Formulations: Water- or oil-dispersible, premix blends, tailored assay

Large-Scale Production Capacity 70,000㎡ facility,6 production workshops, 10 production lines, annual output 6,000+ tons We support large-volume, stable, and long-term supply for global brands.	Advanced Fermentation Technology Clean extraction without harmful solvents Precise control of MK-7 trans isomer ratio High purity and consistent quality batch-to-batch
Strict Quality Control Raw material traceability Pesticide residue testing (EU & US standards) Heavy metals control Stability testing Microbial control	Regulatory & Documentation Support We provide: Certificate of Analysis (COA) Non-GMO statement Allergen-free statement Stability data Technical data sheet Third-party test reports (upon request)

ByAir

100kg-1000kg, 5-7Days

Airport-to-airport service professional

clearance broker needed

BySea

Over300kg, Around 30 Days

Port-to-port service professional

clearance broker needed

Express

Under 100kg, 3-5Days

Door-to-door service easy to pick up the goods

Jiuyuan Biotech supplies Vitamin K2 Powder to food manufacturers, supplement brands, and contract formulators across North America, Europe, and the Asia-Pacific region. Our technical team - not a generic sales inbox - handles every inquiry.

Email: info@jiuyuanbio.com

Website: www.jiuyuanbio.com

Request: Sample| Bulk Quote | CoA & Spec Sheet | Custom Carrier Ratio

Standard response time: within 1 business day. Please include your target application and annual volume estimate for the most relevant technical recommendation.